ITPA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Inosine triphosphatase

Gene Aliases

C20orf37; DEE35; dJ794I6.3; epididymis secretory sperm binding protein; HLC14-06-P; inosine triphosphatase (nucleoside triphosphate pyrophosphatase) ; inosine triphosphate pyrophosphatase; inosine triphosphate pyrophosphohydrolase; ITPase; My049; non-canonical purine NTP pyrophosphatase; non-standard purine NTP pyrophosphatase; NTPase; nucleoside-triphosphate diphosphatase; Nucleoside-triphosphate pyrophosphatase; putative oncogene protein HLC14-06-P.

Gene ID

3704

Accession Number

NP_001254552.1

Reactivity

Homo sapiens, Human

Target

Inosine triphosphate pyrophosphatase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ITPA ELISA Kit (Human) (OKEH04867) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITPA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITPA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol