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CHAT ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Choline O-acetyltransferase

Gene Aliases

Acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6.

Gene ID

1103

Accession Number

NP_001136401.1

Reactivity

Homo sapiens, Human

Target

Choline O-acetyltransferase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CHAT ELISA Kit (Human) (OKEH04859) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHAT captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.088 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

83 kDa

Specificity

Natural and recombinant Human Choline O-acetyltransferase

Protein Length

748

NCBI Gene Symbol

CHAT

Protein Name

P28329

Gene Name URL

CHAT

Nucleotide Accession Number

NM_001142929.1

Frequently Asked Questions

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