IMPA1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Inositol monophosphatase 1
Gene Aliases
D-galactose 1-phosphate phosphatase; IMP; IMP 1; IMPA; IMPase 1; inositol monophosphatase 1; inositol (myo) -1 (or 4) -monophosphatase 1; inositol-1 (or 4) -monophosphatase 1; lithium-sensitive myo-inositol monophosphatase A1; MRT59; myo-inositol monophosphatase 1; testicular tissue protein Li 94.
Gene ID
3612
Accession Number
NP_001138350.1
Reactivity
Homo sapiens, Human
Target
Inositol monophosphatase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IMPA1 ELISA Kit (Human) (OKEH04847) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IMPA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IMPA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IMPA1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
31.2-2000pg/mL
Sensitivity
12 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 kDa
Specificity
Natural and recombinant Human Inositol monophosphatase 1
Protein Length
277
NCBI Gene Symbol
IMPA1
Protein Name
P29218
Gene Name URL
IMPA1
Nucleotide Accession Number
NM_001144878.1
Frequently Asked Questions
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