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S100a4 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

S100 calcium-binding protein A4

Gene Aliases

18A2;42A; calvasculin; CAPL; metastasin; MTS1; nerve growth factor-induced protein 42A; P9K; P9ka; PEL98; placental calcium-binding protein; Protein 9 Ka homologous to calcium-binding protein; protein S100-A4; RNP9KA; S100 calcium-binding protein A4.

Gene ID

24615

Accession Number

NP_036750.1

Reactivity

Rat, Rattus norvegicus

Target

Protein S100-A4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology S100a4 ELISA Kit (Rat) (OKEH04843) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100a4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

7.8-500pg/ml

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

12 kDa

Specificity

Natural and recombinant Rat Protein S100-A4

Protein Length

101

NCBI Gene Symbol

S100a4

Protein Name

P05942

Gene Name URL

S100a4

Nucleotide Accession Number

NM_012618.2

Frequently Asked Questions

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