FGFBP1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor binding protein 1
Gene Aliases
17 kDa HBGF-binding protein;17 kDa heparin-binding growth factor-binding protein; FGF-binding protein 1; FGFBP; FGF-BP; FGFBP-1; FGF-BP1; fibroblast growth factor-binding protein 1; HBP17; heparin-binding growth factor binding protein.
Gene ID
9982
Accession Number
NP_005121.1
Reactivity
Homo sapiens, Human
Target
Fibroblast growth factor-binding protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FGFBP1 ELISA Kit (Human) (OKEH04842) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGFBP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGFBP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Specificity
Natural and recombinant Human Fibroblast growth factor-binding protein 1
Protein Length
234
NCBI Gene Symbol
FGFBP1
Protein Name
Q14512
Gene Name URL
FGFBP1
Nucleotide Accession Number
NM_005130.4
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