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ADCYAP1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Adenylate cyclase activating polypeptide 1

Gene Aliases

Adenylate cyclase activating polypeptide 1 (pituitary) ; PACAP; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP.

Gene ID

116

Accession Number

NP_001093203.1

Reactivity

Homo sapiens, Human

Target

Pituitary adenylate cyclase-activating polypeptide

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ADCYAP1 ELISA Kit (Human) (OKEH04828) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADCYAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADCYAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADCYAP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

7.8-500pg/mL

Sensitivity

2.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Human Pituitary adenylate cyclase-activating polypeptide

Protein Length

176

NCBI Gene Symbol

ADCYAP1

Protein Name

P18509

Gene Name URL

ADCYAP1

Nucleotide Accession Number

NM_001099733.1

Frequently Asked Questions

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