MYH11 ELISA Kit (Human)
Product Specifications
Gene Name
Myosin heavy chain 11
Gene Aliases
AAT4; epididymis secretory sperm binding protein; FAA4; Myosin heavy chain 11; Myosin heavy chain, smooth muscle isoform; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; myosin-11; SMHC; SMMHC; VSCM2.
Gene ID
4629
Accession Number
NP_001035202.1
Reactivity
Homo sapiens, Human
Target
Myosin-11
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MYH11 ELISA Kit (Human) (OKEH04826) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYH11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYH11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYH11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/mL
Sensitivity
0.25 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
227 kDa
Specificity
Natural and recombinant Human Myosin-11
Protein Length
1972
NCBI Gene Symbol
MYH11
Protein Name
P35749
Gene Name URL
MYH11
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001040113.1
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