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MAGEA1 ELISA Kit (Human)

Selected Variant

OKEH04825-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

MAGE family member A1

Gene Aliases

Antigen MZ2-E; cancer/testis antigen 1.1; cancer/testis antigen family 1, member 1; CT1.1; MAGE1; MAGE-1 antigen; melanoma antigen family A 1; melanoma antigen family A, 1 (directs expression of antigen MZ2-E) ; melanoma antigen family A1; melanoma antigen MAGE-1; melanoma-associated antigen 1; melanoma-associated antigen MZ2-E.

Gene ID

4100

Accession Number

NP_004979.3

Reactivity

Homo sapiens, Human

Target

Melanoma-associated antigen 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAGEA1 ELISA Kit (Human) (OKEH04825) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAGEA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAGEA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAGEA1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.625-40ng/mL

Sensitivity

0.326 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 kDa

Specificity

Natural and recombinant Human Melanoma-associated antigen 1

Protein Length

309

NCBI Gene Symbol

MAGEA1

Protein Name

P43355

Gene Name URL

MAGEA1

Nucleotide Accession Number

NM_004988.4

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