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Adra1a ELISA Kit (Rat)

Selected Variant

OKEH04818-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Adrenoceptor alpha 1A

Gene Aliases

Adra1c; adrenergic alpha 1c receptor; adrenergic receptor alpha 1c subtype; adrenergic receptor, alpha 1a; adrenergic receptor, alpha 1c; adrenergic, alpha-1A-, receptor; alpha-1A adrenergic receptor; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; alpha-1C adrenergic receptor.

Gene ID

29412

Accession Number

NP_058887.2

Reactivity

Rat, Rattus norvegicus

Target

Alpha-1A adrenergic receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Adra1a ELISA Kit (Rat) (OKEH04818) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adra1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adra1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adra1a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.087 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

52 kDa

Specificity

Natural and recombinant Rat Alpha-1A adrenergic receptor

Protein Length

466

NCBI Gene Symbol

Adra1a

Protein Name

P43140

Gene Name URL

Adra1a

Nucleotide Accession Number

NM_017191.2

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