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EZR ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Ezrin

Gene Aliases

CVIL; CVL; Cytovillin; cytovillin 2; epididymis secretory protein Li 105; ezrin; HEL-S-105; p81; VIL2; villin 2 (ezrin) ; Villin-2.

Gene ID

7430

Accession Number

NP_001104547.1

Reactivity

Homo sapiens, Human

Target

Ezrin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EZR ELISA Kit (Human) (OKEH04817) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EZR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EZR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EZR captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

31.2-2000pg/mL

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

69 kDa

Specificity

Natural and recombinant Human Ezrin

Protein Length

586

NCBI Gene Symbol

EZR

Protein Name

P15311

Gene Name URL

EZR

Nucleotide Accession Number

NM_001111077.1

Frequently Asked Questions

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