What is the catalog number for Hras1 ELISA Kit (Mouse)?

The catalog number for Hras1 ELISA Kit (Mouse) is OKEH04813-96W.

What sizes are available for Hras1 ELISA Kit (Mouse)?

Hras1 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is Hras1 ELISA Kit (Mouse) in stock?

Hras1 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Hras1 ELISA Kit (Mouse)

Selected Variant

OKEH04813-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Harvey rat sarcoma virus oncogene

Gene Aliases

C-H-; c-Ha-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-H-ras; c-r; c-rasHa; GTPase HRas; H-; Ha; Ha-ras; Harv; Harvey rat sarcoma virus oncogene 1; Harvey-ras; Hr; Hra; H-ras; H-ras 1 protein; Hras1; Hras-1; H-Ras-1; Kras2; p21ras; ras; transforming protein P21.

Gene ID

15461

Accession Number

NP_001123915.1

Reactivity

Mouse, Mus musculus

Target

GTPase HRas

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hras1 ELISA Kit (Mouse) (OKEH04813) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hras1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hras1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hras1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Sensitivity

0.081 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

21 kDa

Specificity

Natural and recombinant Mouse GTPase HRas

Protein Length

189

NCBI Gene Symbol

Hras

Protein Name

Q61411

Gene Name URL

Hras

Nucleotide Accession Number

NM_001130443.1

Price

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