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Ppy ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Pancreatic polypeptide

Gene Aliases

AV061413; I; IGSPP; Pancreatic polypeptide; pancreatic prohormone; PP.

Gene ID

19064

Accession Number

NP_032944.1

Reactivity

Mouse, Mus musculus

Target

Pancreatic prohormone

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ppy ELISA Kit (Mouse) (OKEH04807) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppy has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppy is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppy captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

31.25-2000pg/ml

Sensitivity

15.64 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Specificity

Natural and recombinant Mouse Pancreatic prohormone

Protein Length

100

NCBI Gene Symbol

Ppy

Protein Name

P10601

Gene Name URL

Ppy

Nucleotide Accession Number

NM_008918.1

Frequently Asked Questions

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