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Hist4h4 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Histone cluster 1, H4h

Gene Aliases

1700024H08Rik; B130044J01Rik; germinal histone H4; Gm11275; H2c8; H4; H4 histone family, member A; H4-16; H4c1; H4c11; H4c12; H4c14; H4c2; H4c3; H4c4; H4c6; H4c8; H4c9; H4f16; Hist1; Hist1h; Hist1h4a; Hist1h4b; Hist1h4c; Hist1h4d; Hist1h4f; Hist1h4h; Hist1h4i; Hist1h4j; Hist1h4k; Hist1h4m; Hist1h4n; Hist2; Hist2h4; Hist4; Hist4h4; histone 1, H4a; histone 1, H4b; histone 1, H4c; histone 1, H4d; histone 1, H4f; histone 1, H4h; histone 1, H4i; histone 1, H4j; histone 1, H4k; histone 2, H4; histone 4, H4; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4c; histone cluster 1, H4d; histone cluster 1, H4f; histone cluster 1, H4h; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 1, H4k; histone cluster 1, H4m; histone cluster 1, H4n; histone cluster 2, H4; histone cluster 4, H4; histone H4; X04652.

Gene ID

100041230, 319155, 319156, 319157, 319158, 319159, 319160, 319161, 320332, 326619, 326620, 69386, 97122

Accession Number

NP_001182350.1

Reactivity

Mouse, Mus musculus

Target

Histone H4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hist4h4 ELISA Kit (Mouse) (OKEH04805) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hist4h4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hist4h4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hist4h4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.093 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Specificity

Natural and recombinant Mouse Histone H4

Protein Length

103

NCBI Gene Symbol

H4c1, H4c11, H4c12, H4c14, H4c17, H4c18, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16

Protein Name

P62806

Gene Name URL

H4c1, H4c11, H4c12, H4c14, H4c17, H4c18, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16

Nucleotide Accession Number

NM_001195421.1

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