CHI3L1 ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
Chitinase 3 like 1
Gene Aliases
39 kDa whey protein;40 kDa secretory signalling glycoprotein; Cartilage glycoprotein 39; cartilage glycoprotein-39; CGP-39; chitinase-3-like protein 1; chitinase-like protein 1; CLP-1; GP-39; signal processing protein; Signal-processing protein; SPC-40; SPS-40; YKL-40.
Gene ID
286869
Accession Number
NP_001073688.1
Reactivity
Bos taurus, Bovine
Target
Chitinase-3-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CHI3L1 ELISA Kit (Bovine) (OKEH04798) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHI3L1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHI3L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHI3L1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/mL
Sensitivity
0.28 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Bovine Chitinase-3-like protein 1
Protein Length
383
NCBI Gene Symbol
CHI3L1
Protein Name
P30922
Gene Name URL
CHI3L1
Nucleotide Accession Number
NM_001080219.1
Frequently Asked Questions
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