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Crhbp ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Corticotropin releasing hormone binding protein

Gene Aliases

Corticotropin-releasing hormone-binding protein.

Gene ID

29625

Accession Number

NP_631922.1

Reactivity

Rat, Rattus norvegicus

Target

Corticotropin-releasing factor-binding protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Crhbp ELISA Kit (Rat) (OKEH04789) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crhbp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Crhbp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crhbp captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

0.084 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Rat Corticotropin-releasing factor-binding protein

Protein Length

322

NCBI Gene Symbol

CRHBP

Protein Name

P24388

Gene Name URL

CRHBP

Nucleotide Accession Number

NM_139183.2

Frequently Asked Questions

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