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GREM1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Gremlin 1, DAN family BMP antagonist

Gene Aliases

C15DUPq; cell proliferation-inducing gene 2 protein; CKTSF1B1; colorectal adenoma and carcinoma 1; CRAC1; CRCS4; cysteine knot superfamily 1, BMP antagonist 1; DAN domain family member 2; DAND2; down-regulated in Mos-transformed cells protein; DRM; DUP15q; GREMLIN; gremlin 1, cysteine knot superfamily, homolog; gremlin 1-like protein; gremlin-1; hereditary mixed polyposis syndrome; HMPS; HMPS1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; MPSH; PIG2.

Gene ID

26585

Accession Number

NP_001178252.1

Reactivity

Homo sapiens, Human

Target

Gremlin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GREM1 ELISA Kit (Human) (OKEH04781) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GREM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GREM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GREM1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

125-8000pg/mL

Sensitivity

0.032 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

21 kDa

Specificity

Natural and recombinant Human Gremlin-1

Protein Length

184

NCBI Gene Symbol

GREM1

Protein Name

O60565

Gene Name URL

GREM1

Nucleotide Accession Number

NM_001191323.1

Frequently Asked Questions

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