GREM1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Gremlin 1, DAN family BMP antagonist
Gene Aliases
C15DUPq; cell proliferation-inducing gene 2 protein; CKTSF1B1; colorectal adenoma and carcinoma 1; CRAC1; CRCS4; cysteine knot superfamily 1, BMP antagonist 1; DAN domain family member 2; DAND2; down-regulated in Mos-transformed cells protein; DRM; DUP15q; GREMLIN; gremlin 1, cysteine knot superfamily, homolog; gremlin 1-like protein; gremlin-1; hereditary mixed polyposis syndrome; HMPS; HMPS1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; MPSH; PIG2.
Gene ID
26585
Accession Number
NP_001178252.1
Reactivity
Homo sapiens, Human
Target
Gremlin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GREM1 ELISA Kit (Human) (OKEH04781) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GREM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GREM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GREM1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
125-8000pg/mL
Sensitivity
0.032 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Gremlin-1
Protein Length
184
NCBI Gene Symbol
GREM1
Protein Name
O60565
Gene Name URL
GREM1
Nucleotide Accession Number
NM_001191323.1
Frequently Asked Questions
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