0610037P05Rik ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Centrosomal protein 20
Gene Aliases
0610037P05Rik; centrosomal protein 20; F; Fgfr1op N-terminal like; FGFR1OP N-terminal-like protein; Fopnl; FOP-related protein of 20 kDa; lisH domain-containing protein C16orf63 homolog; lisH domain-containing protein FOPNL.
Gene ID
66086
Accession Number
NP_079621.1
Reactivity
Mouse, Mus musculus
Target
LisH domain-containing protein FOPNL
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology 0610037P05Rik ELISA Kit (Mouse) (OKEH04768) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for 0610037P05Rik has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for 0610037P05Rik is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample 0610037P05Rik captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/ml
Sensitivity
0.408 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 kDa
Specificity
Natural and recombinant Mouse LisH domain-containing protein FOPNL
Protein Length
174
NCBI Gene Symbol
Cep20
Protein Name
Q9CZS3
Gene Name URL
Cep20
Nucleotide Accession Number
NM_025345.2
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