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FOPNL ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Centrosomal protein 20

Gene Aliases

C16orf63; centrosomal protein 20; FGFR1OP N-terminal like; FGFR1OP N-terminal-like protein; FOPNL; FOP-related protein of 20 kDa; FOR20; lisH domain-containing protein C16orf63; lisH domain-containing protein FOPNL; PHSECRG2; pluripotent embryonic stem cell-related protein.

Gene ID

123811

Accession Number

NP_001291426.1

Reactivity

Homo sapiens, Human

Target

LisH domain-containing protein FOPNL

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FOPNL ELISA Kit (Human) (OKEH04767) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOPNL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FOPNL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOPNL captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/ml

Sensitivity

0.174 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

20 kDa

Specificity

Natural and recombinant Human LisH domain-containing protein FOPNL

Protein Length

174

NCBI Gene Symbol

CEP20

Protein Name

Q96NB1

Gene Name URL

CEP20

Nucleotide Accession Number

NM_001304497.1

Frequently Asked Questions

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