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EPHX4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Epoxide hydrolase 4

Gene Aliases

ABHD7; abhydrolase domain containing 7; abhydrolase domain-containing protein 7; EH4; EPHXRP; epoxide hydrolase 4; epoxide hydrolase-related protein.

Gene ID

253152

Accession Number

NP_775838.3

Reactivity

Homo sapiens, Human

Target

Epoxide hydrolase 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology EPHX4 ELISA Kit (Human) (OKEH04764) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human EPHX4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated EPHX4 and incubated. The EPHX4 found in the sample or standards competes with the biotinylated EPHX4 for limited binding sites on the immobilized anti-human EPHX4 antibody. Excess unbound biotinylated EPHX4 and sample or standard EPHX4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated EPHX4 captured in the well and inversely proportional to the amount of EPHX4 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/ml

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Epoxide hydrolase 4

Protein Length

362

NCBI Gene Symbol

EPHX4

Protein Name

Q8IUS5

Gene Name URL

EPHX4

Nucleotide Accession Number

NM_173567.4

Frequently Asked Questions

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