EPHX4 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Epoxide hydrolase 4
Gene Aliases
ABHD7; abhydrolase domain containing 7; abhydrolase domain-containing protein 7; EH4; EPHXRP; epoxide hydrolase 4; epoxide hydrolase-related protein.
Gene ID
253152
Accession Number
NP_775838.3
Reactivity
Homo sapiens, Human
Target
Epoxide hydrolase 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology EPHX4 ELISA Kit (Human) (OKEH04764) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human EPHX4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated EPHX4 and incubated. The EPHX4 found in the sample or standards competes with the biotinylated EPHX4 for limited binding sites on the immobilized anti-human EPHX4 antibody. Excess unbound biotinylated EPHX4 and sample or standard EPHX4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated EPHX4 captured in the well and inversely proportional to the amount of EPHX4 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Epoxide hydrolase 4
Protein Length
362
NCBI Gene Symbol
EPHX4
Protein Name
Q8IUS5
Gene Name URL
EPHX4
Nucleotide Accession Number
NM_173567.4
Frequently Asked Questions
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