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MMEL1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Membrane metalloendopeptidase like 1

Gene Aliases

Membrane metallo-endopeptidase-like 1; membrane metallo-endopeptidase-like 2; MMEL2; NEP2; NEP2 (m) ; NEPII; neprilysin II; neprilysin-2; NL1; NL2; SEP; soluble secreted endopeptidase; zinc metallopeptidase.

Gene ID

79258

Accession Number

NP_258428.2

Reactivity

Homo sapiens, Human

Target

Membrane metallo-endopeptidase-like 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MMEL1 ELISA Kit (Human) (OKEH04755) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMEL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMEL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMEL1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

31.2-2000pg/ml

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

89 kDa

Specificity

Natural and recombinant Human Membrane metallo-endopeptidase-like 1

Protein Length

779

NCBI Gene Symbol

MMEL1

Protein Name

Q495T6

Gene Name URL

MMEL1

Nucleotide Accession Number

NM_033467.3

Frequently Asked Questions

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