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UNC13C ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Unc-13 homolog C

Gene Aliases

Munc13-3; protein unc-13 homolog C; unc-13-like 3.

Gene ID

440279

Accession Number

NP_001074003.1

Reactivity

Homo sapiens, Human

Target

Protein unc-13 homolog C

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology UNC13C ELISA Kit (Human) (OKEH04752) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UNC13C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UNC13C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UNC13C captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

6.25-400pg/mL

Sensitivity

1.56 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

251 kDa

Specificity

Natural and recombinant Human Protein unc-13 homolog C

Protein Length

2214

NCBI Gene Symbol

UNC13C

Protein Name

Q8NB66

Gene Name URL

UNC13C

Nucleotide Accession Number

NM_001080534.1

Frequently Asked Questions

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