Apob ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Apolipoprotein B
Gene Aliases
AI315052; Apo B-100; apob-; apob-100; apob-48; apolipoprotein B-100.
Gene ID
238055
Accession Number
NP_033823.2
Reactivity
Mouse, Mus musculus
Target
Apolipoprotein B-100
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Apob ELISA Kit (Mouse) (OKEH04750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apob has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apob is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apob captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
31.2-2000pg/mL
Sensitivity
15.78 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
509 kDa
Specificity
Natural and recombinant Mouse Apolipoprotein B-100
Protein Length
4505
NCBI Gene Symbol
Apob
Protein Name
E9Q414
Gene Name URL
Apob
Nucleotide Accession Number
NM_009693.2
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