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Prkacb ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Protein kinase, cAMP dependent, catalytic, beta

Gene Aliases

CAMP-dependent protein kinase C beta; cAMP-dependent protein kinase catalytic subunit beta; Cbeta2 PKA; CbPKA; Pka; PKA C-beta; Pkacb.

Gene ID

18749

Accession Number

NP_001157670.1

Reactivity

Mouse, Mus musculus

Target

CAMP-dependent protein kinase catalytic subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Prkacb ELISA Kit (Mouse) (OKEH04743) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prkacb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prkacb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prkacb captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.156-10ng/ml

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

41 kDa

Specificity

Natural and recombinant Mouse cAMP-dependent protein kinase catalytic subunit beta

Protein Length

351

NCBI Gene Symbol

Prkacb

Protein Name

P68181

Gene Name URL

Prkacb

Nucleotide Accession Number

NM_001164198.1

Frequently Asked Questions

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