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Ms4a2 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Membrane spanning 4-domains A2

Gene Aliases

Beta polypeptide) ; fc epsilon receptor I beta-chain; Fcer1b; fcERI; FCIGA; high affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; Membrane-spanning 4-domains subfamily A member 2; Membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2 (Fc fragment of IgE, high affinity I, receptor for; membrane-spanning 4-domains, subfamily A, member 2 (Fc fragment of IgE, high affinity I, receptor for; beta polypeptide) ; Ms4a1.

Gene ID

25316

Accession Number

NP_036977.1

Reactivity

Rat, Rattus norvegicus

Target

High affinity immunoglobulin epsilon receptor subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ms4a2 ELISA Kit (Rat) (OKEH04733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ms4a2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ms4a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ms4a2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

78-5000pg/ml

Sensitivity

41.5 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

27 kDa

Specificity

Natural and recombinant Rat High affinity immunoglobulin epsilon receptor subunit beta

Protein Length

243

NCBI Gene Symbol

Ms4a2

Protein Name

P13386

Gene Name URL

Ms4a2

Nucleotide Accession Number

NM_012845.1

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