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ROCK2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Rho associated coiled-coil containing protein kinase 2

Gene Aliases

P164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; ROCK-II.

Gene ID

9475

Accession Number

NP_001308572.1

Reactivity

Homo sapiens, Human

Target

Rho-associated protein kinase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ROCK2 ELISA Kit (Human) (OKEH04726) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROCK2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROCK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROCK2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

1.56-100ng/mL

Sensitivity

0.76 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

161 kDa

Specificity

Natural and recombinant Human Rho-associated protein kinase 2

Protein Length

1388

NCBI Gene Symbol

ROCK2

Protein Name

O75116

Gene Name URL

ROCK2

Nucleotide Accession Number

NM_001321643.1

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