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CHRM3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cholinergic receptor muscarinic 3

Gene Aliases

Acetylcholine receptor, muscarinic 3; EGBRS; HM3; m3 muscarinic receptor; muscarinic acetylcholine receptor M3; PBS.

Gene ID

1131

Accession Number

NP_000731.1

Reactivity

Homo sapiens, Human

Target

Muscarinic acetylcholine receptor M3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CHRM3 ELISA Kit (Human) (OKEH04721) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRM3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRM3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRM3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

66 kDa

Specificity

Natural and recombinant Human Muscarinic acetylcholine receptor M3

Protein Length

590

NCBI Gene Symbol

CHRM3

Protein Name

P20309

Gene Name URL

CHRM3

Nucleotide Accession Number

NM_000740.2

Frequently Asked Questions

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