CHRM3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cholinergic receptor muscarinic 3
Gene Aliases
Acetylcholine receptor, muscarinic 3; EGBRS; HM3; m3 muscarinic receptor; muscarinic acetylcholine receptor M3; PBS.
Gene ID
1131
Accession Number
NP_000731.1
Reactivity
Homo sapiens, Human
Target
Muscarinic acetylcholine receptor M3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CHRM3 ELISA Kit (Human) (OKEH04721) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRM3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRM3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRM3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
66 kDa
Specificity
Natural and recombinant Human Muscarinic acetylcholine receptor M3
Protein Length
590
NCBI Gene Symbol
CHRM3
Protein Name
P20309
Gene Name URL
CHRM3
Nucleotide Accession Number
NM_000740.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog