What is the catalog number for Shh ELISA Kit (Rat)?

The catalog number for Shh ELISA Kit (Rat) is OKEH04719-96W.

What sizes are available for Shh ELISA Kit (Rat)?

Shh ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is Shh ELISA Kit (Rat) in stock?

Shh ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

Shh ELISA Kit (Rat)

Selected Variant

OKEH04719-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Sonic hedgehog signaling molecule

Gene Aliases

Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; sonic hedgehog homolog; sonic hedgehog protein.

Gene ID

29499

Accession Number

NP_058917.1

Reactivity

Rat, Rattus norvegicus

Target

Sonic hedgehog protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Shh ELISA Kit (Rat) (OKEH04719) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Shh has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Shh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Shh captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

31.2-2000pg/mL

Sensitivity

15.69 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

48 kDa

Specificity

Natural and recombinant Rat Sonic hedgehog protein

Protein Length

437

NCBI Gene Symbol

Shh

Protein Name

Q63673

Gene Name URL

Shh

Nucleotide Accession Number

NM_017221.1

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Anti-CD34 Antibody Picoband®, APC Conjugate

PB9053-APC 100 µg/Vial