Ear11 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Eosinophil cationic protein-like, ribonuclease A family member 3
Gene Aliases
EAR; Ear11; EAR-11; ECP; eosinophil cationic protein; eosinophil secondary granule ribonuclease 11; eosinophil-associated ribonuclease; eosinophil-associated, ribonuclease A family, member 11; R1; R7; ribonuclease 1; ribonuclease 3; ribonuclease 7; ribonuclease, RNase A family, 3; RNase 3.
Gene ID
100361866, 192264
Accession Number
NP_620257.1
Reactivity
Rat, Rattus norvegicus
Target
Eosinophil cationic protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ear11 ELISA Kit (Rat) (OKEH04700) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ear11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ear11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ear11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
62.5-4000pg/mL
Sensitivity
31.28 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 kDa
Specificity
Natural and recombinant Rat Eosinophil cationic protein
Protein Length
155
NCBI Gene Symbol
LOC100361866, Rnase3
Protein Name
P70709
Gene Name URL
LOC100361866, Rnase3
Nucleotide Accession Number
NM_138902.1
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