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CPA3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Carboxypeptidase A3

Gene Aliases

Carboxypeptidase A3; carboxypeptidase A3 (mast cell) ; mast cell carboxypeptidase A; MC-CPA; tissue carboxypeptidase A.

Gene ID

1359

Accession Number

NP_001861.2

Reactivity

Homo sapiens, Human

Target

Mast cell carboxypeptidase A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CPA3 ELISA Kit (Human) (OKEH04688) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CPA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPA3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Sensitivity

0.34 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 KDa

Specificity

Natural and recombinant Human Mast cell carboxypeptidase A

Protein Length

417

NCBI Gene Symbol

CPA3

Protein Name

P15088

Gene Name URL

CPA3

Nucleotide Accession Number

NM_001870.3

Frequently Asked Questions

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