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CLCF1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cardiotrophin like cytokine factor 1

Gene Aliases

B-cell stimulatory factor 3; B-cell-stimulating factor 3; BSF3; BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT1; NNT-1; novel neurotrophin-1; NR6.

Gene ID

23529

Accession Number

NP_001159684.1

Reactivity

Homo sapiens, Human

Target

Cardiotrophin-like cytokine factor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CLCF1 ELISA Kit (Human) (OKEH04677) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLCF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CLCF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLCF1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

25 KDa

Specificity

Natural and recombinant Human Cardiotrophin-like cytokine factor 1

Protein Length

225

NCBI Gene Symbol

CLCF1

Protein Name

Q9UBD9

Gene Name URL

CLCF1

Nucleotide Accession Number

NM_001166212.1

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