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ALDOA ELISA Kit (Human)

Selected Variant

OKEH04675-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldolase, fructose-bisphosphate A

Gene Aliases

ALDA; aldolase A, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; fructose-bisphosphate aldolase A; GSD12; HEL-S-87p; lung cancer antigen NY-LU-1; muscle-type aldolase.

Gene ID

226

Accession Number

NP_000025.1

Reactivity

Homo sapiens, Human

Target

Fructose-bisphosphate aldolase A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ALDOA ELISA Kit (Human) (OKEH04675) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDOA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ALDOA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDOA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma , Urine , tissue homogenates and Cell culture supernates and Other biological fluids

Detection Range

1.56-100U/mL

Sensitivity

0.78U/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

39 KDa

Specificity

Natural and recombinant Human Fructose-bisphosphate aldolase A

Protein Length

364

NCBI Gene Symbol

ALDOA

Protein Name

P04075

Gene Name URL

ALDOA

Nucleotide Accession Number

NM_000034.3

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