RBL1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
RB transcriptional corepressor like 1
Gene Aliases
107 kDa retinoblastoma-associated protein; CP107; p107; PRB1; retinoblastoma-like 1; retinoblastoma-like protein 1.
Gene ID
5933
Accession Number
NP_000312.1
Reactivity
Homo sapiens, Human
Target
Retinoblastoma-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RBL1 ELISA Kit (Human) (OKEH04662) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RBL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for RBL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RBL1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.98 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
121 KDa
Specificity
Natural and recombinant Human Retinoblastoma-like protein 1
Protein Length
1068
NCBI Gene Symbol
RBL1
Protein Name
P28749
Gene Name URL
RBL1
Nucleotide Accession Number
NC_000011.8
Frequently Asked Questions
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