Comp ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cartilage oligomeric matrix protein
Gene Aliases
Cartilage oligomeric matrix protein; thrombospondin-5; TS; TSP5.
Gene ID
12845
Accession Number
NP_057894.2
Reactivity
Mouse, Mus musculus
Target
Cartilage oligomeric matrix protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Comp ELISA Kit (Mouse) (OKEH04657) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Comp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Comp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Comp captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.158 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
82 KDa
Specificity
Natural and recombinant Mouse Cartilage oligomeric matrix protein
Protein Length
755
NCBI Gene Symbol
Comp
Protein Name
Q9R0G6
Gene Name URL
Comp
Nucleotide Accession Number
NM_016685.2
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