Psmb9 ELISA Kit (Rat)
Product Specifications
Gene Name
Proteasome 20S subunit beta 9
Gene Aliases
Large multifunctional protease 2; Lmp2; low molecular mass polypeptide 2; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) ; proteasome (prosome, macropain) subunit, beta type, 9; proteasome chain 7; proteasome subunit beta 9; proteasome subunit beta type-9; proteasome subunit beta-1i; proteosome (prosome, macropain) subunit, beta type 9; really interesting new gene 12 protein; RING12 protein.
Gene ID
24967
Accession Number
NP_036840.2
Reactivity
Rat, Rattus norvegicus
Target
Proteasome subunit beta type-9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Psmb9 ELISA Kit (Rat) (OKEH04655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psmb9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Psmb9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psmb9 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Sensitivity
0.166 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
23 KDa
Specificity
Natural and recombinant Rat Proteasome subunit beta type-9
Protein Length
219
NCBI Gene Symbol
Psmb9
Protein Name
P28077
Gene Name URL
Psmb9
CAS Number
7732-18-5
Nucleotide Accession Number
NM_012708.2
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