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Psmb9 ELISA Kit (Rat)

Product Specifications

Gene Name

Proteasome 20S subunit beta 9

Gene Aliases

Large multifunctional protease 2; Lmp2; low molecular mass polypeptide 2; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) ; proteasome (prosome, macropain) subunit, beta type, 9; proteasome chain 7; proteasome subunit beta 9; proteasome subunit beta type-9; proteasome subunit beta-1i; proteosome (prosome, macropain) subunit, beta type 9; really interesting new gene 12 protein; RING12 protein.

Gene ID

24967

Accession Number

NP_036840.2

Reactivity

Rat, Rattus norvegicus

Target

Proteasome subunit beta type-9

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Psmb9 ELISA Kit (Rat) (OKEH04655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psmb9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Psmb9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psmb9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Sensitivity

0.166 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

23 KDa

Specificity

Natural and recombinant Rat Proteasome subunit beta type-9

Protein Length

219

NCBI Gene Symbol

Psmb9

Protein Name

P28077

Gene Name URL

Psmb9

CAS Number

7732-18-5

Nucleotide Accession Number

NM_012708.2

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