CXCR5 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
C-X-C motif chemokine receptor 5
Gene Aliases
BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5) ; Burkitt lymphoma receptor 1, GTP-binding protein; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR-5; CXC-R5; MDR15; MDR-15; monocyte-derived receptor 15.
Gene ID
643
Accession Number
NP_001707.1
Reactivity
Homo sapiens, Human
Target
C-X-C chemokine receptor type 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CXCR5 ELISA Kit (Human) (OKEH04652) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CXCR5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 KDa
Specificity
Natural and recombinant Human C-X-C chemokine receptor type 5
Protein Length
372
NCBI Gene Symbol
CXCR5
Protein Name
P32302
Gene Name URL
CXCR5
Nucleotide Accession Number
NM_001716.4
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