PDE10A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phosphodiesterase 10A
Gene Aliases
3',5' cAMP/cGMP phosphodiesterase; ADSD2; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dJ416F21.1 (phosphodiesterase 10A) ; HSPDE10A; IOLOD; LINC00473; PDE10A19; phosphodiesterase 10A1 (PDE10A1) ; phosphodiesterase 10A7 (PDE10A7) .
Gene ID
10846
Accession Number
NP_001124162.1
Reactivity
Homo sapiens, Human
Target
CAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDE10A ELISA Kit (Human) (OKEH04648) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE10A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PDE10A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE10A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
88 KDa
Specificity
Natural and recombinant Human cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Protein Length
799
NCBI Gene Symbol
PDE10A
Protein Name
Q9Y233
Gene Name URL
PDE10A
Nucleotide Accession Number
NM_001130690.2
Frequently Asked Questions
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