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DDO ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

D-aspartate oxidase

Gene Aliases

Aspartic oxidase; DASOX; D-aspartate oxidase; D-aspartate oxidase, DDO; DDO-1; DDO-2.

Gene ID

8528

Accession Number

NP_003640.2

Reactivity

Homo sapiens, Human

Target

D-aspartate oxidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DDO ELISA Kit (Human) (OKEH04646) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for DDO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDO captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.11 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 KDa

Specificity

Natural and recombinant Human D-aspartate oxidase

Protein Length

341

NCBI Gene Symbol

DDO

Protein Name

Q99489

Gene Name URL

DDO

Nucleotide Accession Number

NM_003649.2

Frequently Asked Questions

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