LGR4 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Leucine rich repeat containing G protein-coupled receptor 4
Gene Aliases
BNMD17; G protein-coupled receptor 48; GPR48; G-protein coupled receptor 48; leucine-rich repeat-containing G-protein coupled receptor 4.
Gene ID
55366
Accession Number
NP_060960.2
Reactivity
Homo sapiens, Human
Target
Leucine-rich repeat-containing G-protein coupled receptor 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LGR4 ELISA Kit (Human) (OKEH04645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGR4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LGR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGR4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
0.32 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
104 KDa
Specificity
Natural and recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 4
Protein Length
951
NCBI Gene Symbol
LGR4
Protein Name
Q9BXB1
Gene Name URL
LGR4
Nucleotide Accession Number
NM_018490.2
Frequently Asked Questions
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