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DCT ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Dopachrome tautomerase

Gene Aliases

Dopachrome delta-isomerase; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) ; DT; L-dopachrome Delta-isomerase; L-dopachrome isomerase; L-dopachrome tautomerase; OCA8; TRP2; TRP-2; tyrosinase related protein-2; Tyrosinase-related protein 2; tyrosine-related protein 2; TYRP2.

Gene ID

1638

Accession Number

NP_001123361.1

Reactivity

Homo sapiens, Human

Target

L-dopachrome tautomerase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DCT ELISA Kit (Human) (OKEH04644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DCT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for DCT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DCT captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

59 KDa

Specificity

Natural and recombinant Human L-dopachrome tautomerase

Protein Length

519

NCBI Gene Symbol

DCT

Protein Name

P40126

Gene Name URL

DCT

Nucleotide Accession Number

NM_001129889.2

Frequently Asked Questions

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