DCT ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Dopachrome tautomerase
Gene Aliases
Dopachrome delta-isomerase; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) ; DT; L-dopachrome Delta-isomerase; L-dopachrome isomerase; L-dopachrome tautomerase; OCA8; TRP2; TRP-2; tyrosinase related protein-2; Tyrosinase-related protein 2; tyrosine-related protein 2; TYRP2.
Gene ID
1638
Accession Number
NP_001123361.1
Reactivity
Homo sapiens, Human
Target
L-dopachrome tautomerase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DCT ELISA Kit (Human) (OKEH04644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DCT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for DCT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DCT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 KDa
Specificity
Natural and recombinant Human L-dopachrome tautomerase
Protein Length
519
NCBI Gene Symbol
DCT
Protein Name
P40126
Gene Name URL
DCT
Nucleotide Accession Number
NM_001129889.2
Frequently Asked Questions
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