MCHR2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Melanin concentrating hormone receptor 2
Gene Aliases
G protein-coupled receptor slt; GPR145; G-protein coupled receptor 145; GPRv17; MCH receptor 2; MCH2; MCH2R; MCH-2R; MCHR-2; MCH-R2; melanin-concentrating hormone 2; melanin-concentrating hormone receptor 2; SLT.
Gene ID
84539
Accession Number
NP_001035269.1
Reactivity
Homo sapiens, Human
Target
Melanin-concentrating hormone receptor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MCHR2 ELISA Kit (Human) (OKEH04642) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MCHR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MCHR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MCHR2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 KDa
Specificity
Natural and recombinant Human Melanin-concentrating hormone receptor 2
Protein Length
340
NCBI Gene Symbol
MCHR2
Protein Name
Q969V1
Gene Name URL
MCHR2
Nucleotide Accession Number
NM_001040179.1
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