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KBTBD13 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Kelch repeat and BTB domain containing 13

Gene Aliases

HCG1645727; kelch repeat and BTB (POZ) domain containing 13; kelch repeat and BTB domain-containing protein 13; NEM6; nemaline myopathy type 6.

Gene ID

390594

Accession Number

NP_001094832.1

Reactivity

Homo sapiens, Human

Target

Kelch repeat and BTB domain-containing protein 13

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology KBTBD13 ELISA Kit (Human) (OKEH04630) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KBTBD13 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for KBTBD13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KBTBD13 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 KDa

Specificity

Natural and recombinant Human Kelch repeat and BTB domain-containing protein 13

Protein Length

458

NCBI Gene Symbol

KBTBD13

Protein Name

C9JR72

Gene Name URL

KBTBD13

Nucleotide Accession Number

NM_001101362.2

Frequently Asked Questions

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