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Nrn1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Neuritin 1

Gene Aliases

0710008J23Rik; candidate plasticity gene 15 protein; candidate plasticity-related gene 15; cpg1; Cpg15; neuritin; Nrn.

Gene ID

68404

Accession Number

NP_705757.1

Reactivity

Mouse, Mus musculus

Target

Neuritin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nrn1 ELISA Kit (Mouse) (OKEH04625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nrn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nrn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nrn1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.179 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 KDa

Specificity

Natural and recombinant Mouse Neuritin

Protein Length

142

NCBI Gene Symbol

Nrn1

Protein Name

Q8CFV4

Gene Name URL

Nrn1

Nucleotide Accession Number

NM_153529.2

Frequently Asked Questions

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