LITAF ELISA Kit (Chicken)

Product Specifications

CAS Number

7732-18-5

Gene Name

Lipopolysaccharide induced TNF factor

Gene Aliases

Lipid-induced TNF-alpha factor; lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; LPS-induced TNF-alpha factor homolog; small integral membrane protein of lysosome/late endosome; tissue necrosis factor-alpha; TNF-alpha; TNF-alpha factor; tumor necrosis factor-alpha.

Gene ID

374125

Accession Number

NP_989598.1

Reactivity

Chicken, Gallus gallus

Target

Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LITAF ELISA Kit (Chicken) (OKEH04620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LITAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LITAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LITAF captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol