LITAF ELISA Kit (Chicken)
Product Specifications
CAS Number
7732-18-5
Gene Name
Lipopolysaccharide induced TNF factor
Gene Aliases
Lipid-induced TNF-alpha factor; lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; LPS-induced TNF-alpha factor homolog; small integral membrane protein of lysosome/late endosome; tissue necrosis factor-alpha; TNF-alpha; TNF-alpha factor; tumor necrosis factor-alpha.
Gene ID
374125
Accession Number
NP_989598.1
Reactivity
Chicken, Gallus gallus
Target
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LITAF ELISA Kit (Chicken) (OKEH04620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LITAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LITAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LITAF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 KDa
Specificity
Natural and recombinant Chicken Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
Protein Length
148
NCBI Gene Symbol
LITAF
Protein Name
Q8QGW7
Gene Name URL
LITAF
Nucleotide Accession Number
NM_204267.1
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