ESR1 ELISA Kit (Chicken)
Product Specifications
CAS Number
7732-18-5
Gene Name
Estrogen receptor 1
Gene Aliases
ER; ER-alpha; estradiol receptor; estrogen receptor; estrogen receptor, alpha; nuclear receptor subfamily 3 group A member 1.
Gene ID
396099
Accession Number
NP_990514.1
Reactivity
Chicken, Gallus gallus
Target
Estrogen receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ESR1 ELISA Kit (Chicken) (OKEH04619) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ESR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ESR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ESR1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 KDa
Specificity
Natural and recombinant Chicken Estrogen receptor
Protein Length
589
NCBI Gene Symbol
ESR1
Protein Name
P06212
Gene Name URL
ESR1
Nucleotide Accession Number
NM_205183.2
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