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SCD ELISA Kit (Pig)

Selected Variant

OKEH04616-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Aliases

Delta (9) -desaturase; Fatty acid desaturase; Stearoyl-CoA desaturase.

Gene ID

396670

Accession Number

NP_998946.1

Reactivity

Porcine, Sus scrofa

Target

Acyl-CoA desaturase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SCD ELISA Kit (Pig) (OKEH04616) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SCD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCD captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.055 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 KDa

Specificity

Natural and recombinant Pig Acyl-CoA desaturase

Protein Length

334

NCBI Gene Symbol

SCD

Protein Name

O02858

Gene Name URL

SCD

Nucleotide Accession Number

NM_213781.1

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