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CXCR2 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Chemokine (C-X-C motif) receptor 1

Gene Aliases

Chemokine (C-X-C) receptor 1; C-X-C chemokine receptor type 1; C-X-C chemokine receptor type 2; CXCR2; CXCR-2; CXC-R2; high affinity interleukin-8 receptor B; IL-8R B; IL8RA; IL8RB; interleukin 8 receptor, alpha; interleukin 8 receptor, beta.

Gene ID

281863

Accession Number

NP_776785.1

Reactivity

Bos taurus, Bovine

Target

C-X-C chemokine receptor type 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CXCR2 ELISA Kit (Bovine) (OKEH04615) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CXCR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.097 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

41 KDa

Specificity

Natural and recombinant Bovine C-X-C chemokine receptor type 2

Protein Length

360

NCBI Gene Symbol

CXCR1

Protein Name

Q28003

Gene Name URL

CXCR1

Nucleotide Accession Number

NM_174360.2

Frequently Asked Questions

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