KITLG ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
KIT ligand
Gene Aliases
C-kit ligand; kit ligand; Mast cell growth factor; mast cell growth factor (white heifer disease) ; MGF; SCF; stem cell factor.
Gene ID
281885
Accession Number
NP_776800.1
Reactivity
Bos taurus, Bovine
Target
Kit ligand
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KITLG ELISA Kit (Bovine) (OKEH04606) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KITLG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for KITLG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KITLG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
30 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 KDa
Specificity
Natural and recombinant Bovine Kit ligand
Protein Length
274
NCBI Gene Symbol
KITLG
Protein Name
Q28132
Gene Name URL
KITLG
Nucleotide Accession Number
NM_174375.2
Frequently Asked Questions
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